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Prøveudtagning

By april 9, 20192018

Prøvetagning – ingen kæde er stærkere end det svageste led!

Hvorfor overhovedet beskæftige sig med prøvetagning i miljøet?

  1. For at forebygge udfordringer med biostammer af uønsket mikrobiologi i produktionsområdet og en potentiel tilbagetrækning af produkt eller syge gæster?
  2. Fordi lovgivningen kræver det?

Det er vigtigt at huske, at man gør det her for at finde noget, hvis det er der, så der kan gøres noget ved det!

Faktorer som spiller ind på resultatet.

  1. Prøvetagningsteknik
  2. Prøvetagningsmaterialet
  3. Den efterfølgende analyse

Hvilke faktorer skal overvejes i valget af prøvetagningsudstyret?

  1. Er det en kvantitativ eller kvalitativ analyse der skal laves efterfølgende.
  2. Hvilket underlag er det der skal testes?
    • Er det et kritisk kontrolpunkt (CCP), et punkt tæt på en fødevarekontaktoverflade eller længere væk, som eksempelvis et afløb.
    • Er overfladen våd eller tør?
    • Er det før eller efter eventuel desinfektion af overfladen.
    • Arealet af overfladen som skal testes.
  3. Hvilken type mikroorganisme er det vi leder efter?
  4. Hvor lang tid går der fra prøven er taget til den analyseres?

Der er hjælp at hente i ISO18593:2018, som angiver metoder til horisontal prøvetagning med svabere, svampe og klude.

Til mindre områder (op til 100 cm2), som også kan være svært tilgængelige anbefales svabere. Hvis prøvetagningsområdet overstiger 100 cm2 anbefales svamp (evt. med håndtag) eller klud.

Hvis overfladen er våd, kan der bruges tørt prøvetagningsudstyr. Ellers anbefales prevædet prøvetagningsudstyr. Væsken kan være sterilt vand, eller eksempelvis peptone buffer. Hvis der kan være rester af desinfektionsmiddel på overfladen, anbefales det ydermere at vælge prøvetagningsudstyr tilsat neutralisatorer.

Uanset hvilken opgave det er du skal løse, er vi klar med rådgivning og det rette produkt. Du er meget velkommen til at kontakte os til en uformel snak om din prøvetagning. Du kan også søge inspiration i vores store udvalg, som der vises udpluk af her nedenfor.

Helle Eis

For mere information kontakt Helle Eis på mobil: 4268 7218 eller helle.eis@fooddiagnostics.dk

Jens Bachmann

For mere information kontakt Jens Bachmann på mobil: 5130 2666 eller jens@fooddiagnostics.dk

World Bioproducts

PUR-Blue Swab Sampler

PUR-Blue™ Swab Sampler

Our PUR-Blue™ Swab Sampler is useful for sampling small and large (up to 1ft2) surface areas for quantitative purposes or for pathogen detection. An independent laboratory study showed that higher counts are obtained with PUR-Blue when compared to other swabs.

PUR-Blue™ Swab, 100/case Varenr.:
1.5 ml D/E Neutralizing Broth BLU-DE-P
1.5 ml HiCap™ Neutralizing Broth BLU-HC-P
1.5 ml Neutralizing Buffer BLU-NB-P
1.5 ml Letheen Broth BLU-LET-P
5 ml Letheen Broth BLU-5LET
10 ml Letheen Broth BLU-10LET
4 ml Neutralizing Buffer BLU-4NB
5 ml Neutralizing Buffer BLU-5NB
10 ml Neutralizing Buffer BLU-10NB
5 ml HiCap™ Neutralizing Broth BLU-5HC
10 ml HiCap™ Neutralizing Broth BLU-10HC
1 ml Letheen Broth, small swab BLU-1LET-S
1 ml HiCap™ Broth, small swab BLU-1HC-S
Dry, sterile swab BLU-DRY

PUR-Blue™ DUO Swab Sampler

PUR-Blue™ DUO Swab Sampler collects and enriches at point of collection. The swab comes pre-moistened with 1.5 ml of collection solution. The second side contains 9 ml of enrichment broth. After sample collection, the swab is immediately added to the broth to start sample enrichment.

PUR-Blue DOU™ Swab, 100/case Varenr.:
1.5 ml HiCap™ / 9 ml UVM DUO-HC9UVM
1.5 ml HiCap™ / 9 ml Demi-Fraser Broth DUO-HC9DFB
1.5 ml HiCap™ / 9 ml Buffered Peptone Water DUO-HC9BPW
1.5 ml D/E / 9 ml UVM DUO-DE9DFB
1.5 ml D/E / 9 ml Demi-Fraser Broth DUO-DE9DFB
1.5 ml D/E / 9 ml Buffered Peptone Water DUO-DE9BPW
PUR-Blue DUP Swab Sampler
EZ Reach Sponge Sampler

EZ Reach™ Sponge Sampler

The EZ Reach™ Sponge Sampler has a single flexible handle that detaches with an easy twist motion. Choose either a robust polyurethane or cellulose sponge and either a 24 oz or 18 oz sample bag.

EZ Reach™ polyurethane, 100/case Varenr.:
10 ml HiCap™ Neutralizing Broth EZ-10HC-PUR
10 ml HiCap™ w/gloves EZ-10HC-PUR-G
10 ml HiCap™ 18 oz bag w/gloves EZ18FR-10HC-PUR
10 ml D/E Neutralizing Broth EZ-10DE-PUR
10 ml Letheen Broth EZ-10LET-PUR
10 ml Letheen Broth, 18 oz bag EZ18FR-10LET-PUR
10 ml Neutralizing Buffer EZ-10NB-PUR
10 ml Neutralizing Buffer w/gloves EZ-10NB-PUR-G
10 ml Neutralizing Buffer 18 oz bag EZ18FR-10NB-PUR
10 ml Neutralizing Buffer 18 oz w/gloves EZ18FR-10NB-PUR-G
10 ml Buffered Peptone Water EZ18FR-10BPW-PUR
Dry, sterole sponge EZ-DRY-PUR
EZ Reach™ cellulose, 100/case Varenr.:
10 ml Letheen Broth EZ-10LET-CELL
10 ml Letheen Broth w/gloves EZ-10LET-CELL-G
10 ml Neutralizing Buffer EZ-10NB-CELL
10 ml Neutralizing Buffer w/gloves EZ-10NB-CELL-G
10 ml Buffered Peptone Water EZ-10BPW-CELL
25 ml Buffered Peptone Water EZ-25BPW-CELL
Dry, sterile cellulose sponge EZ-DRY-CELL
Adapters & Extension Poles Varenr.:
EZ Reach™ Sponge Adapter for poles, each EZ-ADAPT
PUR-Blue™ Swab Adapter for poles, each BLU-ADAPT
4 ft Extension Pole, each EXT POLE 4-FT
8 ft Extension Pole, each EXT POLE 8-FT
EZ-Reach Split Sampler

EZ Reach™ Split-Sampler

The EZ Reach™ Split-Sampler is our newest innovation, designed to deliver 2 samples form 1 collection site.

EZ Reach™ Split-Sampler, 60/case Varenr.:
20 ml HiCap™ Neutralizing Broth EZ-SP-20HC-PUR
20 ml D/E Netralizing Broth EZ-SP-20DE-PUR
20 ml Letheen Broth EZ-SP-20LET-PUR
20 ml Neutralizing Broth EZ-SP-20NB-PUR

SampleRight™ Sponge Sampler

Our SampleRight™ Sponge Sampler is a traditional sponge sampling device for enviromental and carcass surfaces it includes a 1.5 x 3″ biocide-free polyurethane or cellulose sponge within a 18 oz or 24 oz sample bag. Each bag is printed with a white write-on area and is available with or without gloves attached.

SampleRight™ polyurethane Varenr.:
16 ml HiCap™ 18 oz bag, 100/case EZ-SP-20HC-PUR
SampleRight™ cellulose, 100/case Varenr.:
10 ml HiCap™ 18 oz bag SR18-10HC
10 ml HiCap™ 18 oz bag w/gloves SR18-10HC-G
10 ml Letheen Broth SR-10LET
10 ml Letheen Broth w/gloves SR-10LET-G
10 ml Letheen Broth, 18 oz bag w/gloves SR18-10LET-G
10 ml Neutralizing Buffer SR-10NB
10 ml Neutralizing Buffer w/gloves SR-10NB-G
10 ml Neutralizing Buffer 18 oz bag w/gloves SR18-10NB-G
10 ml Buffered Peptone Water w/gloves SR-10BPW-G
10 ml Buffered Peptone Water 18 oz bag SR18-10BPW
10 ml Buffered Peptone Water 18 oz w/gloves SR18-10BPW-G
Dry cellulose sponge SR-DRY
Dry cellulose sponge w/gloves SR-DRY-G
Dry cellulose sponge, 18 oz bag SR18-DRY
Dry cellulose sponge, 18 oz w/gloves SR18-DRY-G
SampleRight Sponge Sampler
Veriswab Swab Sampler

Veriswab™ Swab Sampler

The Veriswab™ Sampler uses a Dacron tipped swab for reaching into small, hard to access areas.

Veriswab™ Swab Sampler Varenr.:
1 ml HiCap™ Neutralizing Broth VS-1HC
10 ml Neutralizing Buffer VS-10NB
4 ml Neutralizing Buffer VS-4NB
10 ml Buffered Peptone Water VS-10BPW
1 ml Letheen Broth VS-1LET
4 ml Letheen Broth VS-4LET
10 ml Letheen Broth VS-10LET

MWE Medical Wire

Wide range of applications throughout food and pharmaceutical manufacturing and processing

Food & Pharmaceutical Manufacturing

Sponge swabs are especially recommended for the sampling of larger areas for the detection of controlled pathogens such as Listeria, Salmonella and Coliforms. They are very useful for sampling irregular surfaces such as drains and machine casings, and also for larger areas such as conveyor belts and preparation benches. Polywipes™ with standard phosphate buffer has been widely used for many years, and is the most suitable formulation for most applications. There are some situations, whether specified by regulations, or the requirements of particular retailers, where alternative wetting solutions are required. To meet these requirements, Polywipes™ are now also available with Neutralising Rinse Solution (NRS™) , Buffered Peptone Water (BPW) or Letheen Broth. NRS™ is a peptone solution containing the neutralisers sodium thiosulphate, polysorbate 80, and lecithin, and is often specified for standard swab sampling. Buffered Peptone Water is recommended where Salmonella is a particular target on food contact surfaces. Letheen Broth is a growth medium which also contains the neutralisers lecithin and polysorbate 80, and is recommended by the FDA for environmental testing for the cosmetic industry. Peptone Saline (also called Maximum Recovery Diluent – MRD) is available as the wetting solution with Polywipes™ Carcass Swabs (see below).

Carcass swabs

European and international standards (including (EC) No. 2073/2005) require the regular sampling of the carcasses of cattle, sheep, pigs, goats and horses using sponge swabs. Polywipes™ Carcass Swabs are premoistened with Peptone Saline specially formulated (ISO 17604 & ISO 6887) to comply with these
regulations. They can be supplied complete with gloves, resealable bags, and preprinted label for recording required details such as Farm, Species, Length and Reference Number.

New

  • l Range of premoistening buffers
  • l Standard (Phosphate buffer)
  • l NRS – neutralising rinse solution
  • l Letheen Broth
  • l BPW- Buffered Peptone Water
  • l Peptone Saline (for carcass swabbing)

Key Features

  • l Sterile – guaranteed by irradiation
  • l Triple packed for controlled handling
  • l Bright blue sponge (highly visible – does not get lost)
  • l Resistant to abrasion
  • l Non-inhibitory – optimum recovery of target microorganisms
  • l Premoistened with choice of buffers
  • l Room temperature storage
  • l Individually wrapped – tamper evident packaging
  • l Variety of formats (gloves, bags, etc)
  • l Scientifically attested

Polywipes™

Premoistened sponge swabs for sampling large areas

Polywipes™ are sterile premoistened blue sponge swabs, ready to use and highly effective for the sampling and microbiological assessment of a wide variety of surfaces.

Polywipes™ are manufactured from a special sponge material containing no inhibitory substances, so that maximum recovery and detection of microorganisms is assured. The sponge format is convenient for sampling larger flat or irregular surfaces, such as conveyor belts and machine parts, as well as floors, walls, drains and ceilings.

Polywipes™ are premoistened with phosphate buffer, or any of a selection of other buffers, and individually sealed in a easy-to-open tamper evident peel pouch. The bright blue colour ensures they cannot be easily lost in critical areas, while the structure within the sponge prevents shedding of fragments due to abrasion.
Polywipes™ are also convenient to store at room temperature, and can be carried individually to the sites for testing. They are triple packed, and terminally sterilised by gamma irradiation.

Using sterile gloves, which can be supplied, the sponge is used to sample the surface being tested. Any traces of disinfectant are quickly neutralised, either by simple dilution, or by direct interaction with the neutralising components of the liquid. After use and transportation, they are readily processed in the laboratory by any of a number of standard techniques.

Polywipes™ have been the subject of many studies, including recovery performance with Salmonella, Listeria, MRSA,
Acinetobacter, and Clostridium difficile.

Polywipes
Transwab

NRSII Transwab©

Neutralises trace disinfectant

NRS II Transwab® is MWE’s name for its range of swab based environmental sampling devices for the food, pharmaceutical, biotechnology and cosmetic industries. There can also be applications in healthcare including the monitoring of infection control measures.

All NRS II Transwab® devices feature MWE’s leak proof labelled self standing screw-cap tube made from shatterproof polypropylene, with a high visibility blue shaft swab attached to the cap. The swab features a rayon bud that can remain immersed in liquid, yet retains MWE’s high standard for absorption, survival and release of microorganisms. The tubes are prefilled with the specified volume of solution and there is a choice of NRS, MWE’s classic neutralising rinse solution, and a range of alternative buffers and media appropriate for particular applications.

NRS (Neutralising Rinse Solution)

NRS Medium contains lecithin, polysorbate 80, and sodium thiosulphate in a peptone phosphate buffer. The formulation conforms to ISO 18593 and will neutralise most disinfectants used in the food industry, including those based on chlorine, peroxygen compounds, amphoterics, biguanides, and glutaraldehyde. This is a universal neutralising solution suitable for testing most disinfected areas within the food, cosmetic and pharmaceutical industries. Precise fill volumes allow accurate quantitative assessment of contamination levels.

Buffered Peptone Water

Buffered Peptone Water contains peptone and sodium chloride, with a phosphate buffer, and is used for the recovery of Salmonella, especially in environments where they may have been sub-lethally injured.

Butterfield Buffer

Butterfield Buffer (also called Butterfield’s Solution or Butterfield’s phosphate buffered dilution water) contains potassium phosphate as specified by APHA and FDA, and is used in standard methods for the enumeration of bacteria and fungi in foods, water and pharmaceutical products. The standard formulation has been modified by the inclusion of a low leve one to reduce osmotic shock, and polysorbate 80 as a surfactant to assist the sampling process.

Letheen Broth

Letheen media are used to determine the bactericidal efficacy of quaternary ammonium based disinfectants. Letheen broth is a growth medium recommended by the FDA for use in the microbiological testing of cosmetics, and the formulation is as described in the FDA Bacteriological Analytical Manual. Polysorbate 80 and lecithin are included to partially neutralise the preservatives commonly found in cosmetics.

MRD (Maximum Recovery Diluent)

Also called Peptone Saline. Maximum Recovery Diluent is an isotonic and protective medium for maximum recovery of microorganisms from environmental and food sources. It is also the recommended diluent for the sampling of carcasses in the European Union.

Tryptic Soy Broth

Tryptic Soy Broth is a general purpose recovery medium with excellent growth properties for aerobes, anaerobes, and some fungi. It is widely used for sterility testing, particularly in pharmaceutical manufacturing.

NRS without Sodium Thiosulphate

Some testing methods (such as Petrifilm®) require medium without thiosulphate.

Choice of fill

NRS medium and variants are available in a choice of 3 fill sizes

  • l 10ml and 5ml are used with standard and filtration methods.
  • l 1ml can be used directly with Petrifilm®, and other direct pour plating techniques

SteriKit™

For sterile manufacturing areas

SteriKit™ is a swab based system for the detection of microbial contamination in sterile production facilities.

Samples are collected using Steriswab™ premoistened swabs, and inserted into the SteriKit™ tubes containing an enriched semi-solid medium with colour change growth indicator. Upon incubation any bacteria will grow and cause the colour of the medium to change from purple to yellow within 48 hours.
The time taken will depend on the number of organisms, but in the event of any colour development, the swab and medium can be used for further investigation by conventional or molecular microbiological techniques.

  • The product is triple wrapped to allow sterile handling in controlled areas, and sterilised by irradiation.
  • Colour-change indication of microbial growth
  • Enriched growth medium for enhanced detection for low numbers
  • Premoistened swab for effective sampling
  • Formulated and tested in accordance with UK, European & US Pharmacopoeias
  • Ambient temperature storage

Flexiswab™

Longer flexible-shafted swab

Flexiswab™ is ideal for taps, pipes, vents, valves, vats, machinery, crevices, and other
hard-to-reach areas.

  • l Strong flexible longer 8 inch/ 205mm shaft for extra reach around bends without breaking.
  • l High visibility blue shaft
  • l Larger rayon bud for effective sampling with good absorption and release.
  • l Tamper evident seal ensures sterility prior to sampling
  • l Labelled tube for transport of sample, and recording site details

Steriswab™

Sampling for critical surfaces

Steriswabs™ are designed for sampling of critical surfaces including nozzles and equipment in sterile production units
in hospitals and in the pharmaceutical industries. They are also particularly useful for surveillance of clean and food contact surfaces in the food manufacturing industry.

Steriswabs™ have a rayon bud on a blue plastic shaft, and are premoistened with a sterile phosphate buffer. The moistened bud improves the recovery of any microorganisms from the test surface, even if present in low numbers. The swabs have a 45mm breakp m to be readily transferred into other test systems.

Steriswabs™ are triple wrapped for sterile hand controlled premises, and are sterilised by irradiation.

PreMoist Swab Sampling Kits

PreMoist sampling swabs offer end-users a convenient and quick sampling method to confirm the presence of bacteria with no additional preparation required. PreMoist Swabs can be used for environmental and hygiene monitoring, food security and cleaning validation due to the inclusion of neutralisers.

In many cases a surface will contain traces of disinfectants and/or sanitisers. Left alone and over time, these cleaning fluid traces will not kill the microorganisms, but will just prevent any growth (bacteriostatic effect). TSC Neutralising Buffer will neutralise most common disinfectants including peroxides and derivatives, phenols, quaternary ammonium compounds (QAC’s), amphoterics, chlorines and associated halogens, providing end-users with total testing assurance.

Surface Sampling Swabs

  • Easy direct sampling
  • Choice of viscose and foam tips on white or blue polystyrene shafts
  • Foam Tip Swab increases sample collection & release
  • Define sampling areas by using a sterile sampling template [TS/15-T40]
  • Labelled tubes for easier sample processing and identification
  • Tamper evident seals & shatterproof tubes offer total product confidence

Sampling Areas

  • Entire sample can be analysed
  • Ideal for sampling a wide range of environments and critical surfaces
  • Exposes potential microorganism contamination
  • Useful in hygiene surveillance (HACCP) and environmental monitoring
  • Food security / TACCP validation
  • Protecting your brand and preventing costly recalls
Quetip

SodiBox

Item 4513 – Cloth 32 x 40 cm, Tryptone salt at 10% neutralizing agent,
in blue sampling bag with pre-marked line at top for easy opening

Use

Sterile device for surface sampling. For screening pathogens after disinfection in food processing industries, especially dairy.

Features

  •  Blue non-woven cloth, honeycombed structure, size 32 x 40 cm
  • Sampling bag l 19,5 x h 29,5 cm with pre-marked line at top for easy opening, one side blue for identification, one side clear
  • Impregnation « TN »: 25 ml Tryptone salt at 10% neutralizing agent.
    The neutralizing agent stops the inhibitory action of residual disinfectants and antiseptics (cf. Product data of Neutralizing agent). Calibrated dose
  • Economical version: pack of 20 units in a zip bag, size 27 x 38 cm

Sales unit

  • Box of 140 units (7 x 20 units)
  • Dimensions of box approx. 40 x 30 x 30 cm
  • Weight of box approx. 7 kg

Sterilization and traceability

  • Sterilization by gamma rays dose 15 kiloGrays minimum
  • Shelf life : 1 year from the date of sterilisation
  • Batch number on each unit and on each box
  • Sterilization certificate in each box
SodiBox