Prøvetagning – ingen kæde er stærkere end det svageste led!
Hvorfor overhovedet beskæftige sig med prøvetagning i miljøet?
- For at forebygge udfordringer med biostammer af uønsket mikrobiologi i produktionsområdet og en potentiel tilbagetrækning af produkt eller syge gæster?
- Fordi lovgivningen kræver det?
Det er vigtigt at huske, at man gør det her for at finde noget, hvis det er der, så der kan gøres noget ved det!
Faktorer som spiller ind på resultatet.
- Den efterfølgende analyse
Hvilke faktorer skal overvejes i valget af prøvetagningsudstyret?
- Er det en kvantitativ eller kvalitativ analyse der skal laves efterfølgende.
- Hvilket underlag er det der skal testes?
- Er det et kritisk kontrolpunkt (CCP), et punkt tæt på en fødevarekontaktoverflade eller længere væk, som eksempelvis et afløb.
- Er overfladen våd eller tør?
- Er det før eller efter eventuel desinfektion af overfladen.
- Arealet af overfladen som skal testes.
- Hvilken type mikroorganisme er det vi leder efter?
- Hvor lang tid går der fra prøven er taget til den analyseres?
Der er hjælp at hente i ISO18593:2018, som angiver metoder til horisontal prøvetagning med svabere, svampe og klude.
Til mindre områder (op til 100 cm2), som også kan være svært tilgængelige anbefales svabere. Hvis prøvetagningsområdet overstiger 100 cm2 anbefales svamp (evt. med håndtag) eller klud.
Hvis overfladen er våd, kan der bruges tørt prøvetagningsudstyr. Ellers anbefales prevædet prøvetagningsudstyr. Væsken kan være sterilt vand, eller eksempelvis peptone buffer. Hvis der kan være rester af desinfektionsmiddel på overfladen, anbefales det ydermere at vælge prøvetagningsudstyr tilsat neutralisatorer.
Uanset hvilken opgave det er du skal løse, er vi klar med rådgivning og det rette produkt. Du er meget velkommen til at kontakte os til en uformel snak om din prøvetagning. Du kan også søge inspiration i vores store udvalg, som der vises udpluk af her nedenfor.
PUR-Blue™ Swab Sampler
Our PUR-Blue™ Swab Sampler is useful for sampling small and large (up to 1ft2) surface areas for quantitative purposes or for pathogen detection. An independent laboratory study showed that higher counts are obtained with PUR-Blue when compared to other swabs.
|PUR-Blue™ Swab, 100/case||Varenr.:|
|1.5 ml D/E Neutralizing Broth||BLU-DE-P|
|1.5 ml HiCap™ Neutralizing Broth||BLU-HC-P|
|1.5 ml Neutralizing Buffer||BLU-NB-P|
|1.5 ml Letheen Broth||BLU-LET-P|
|5 ml Letheen Broth||BLU-5LET|
|10 ml Letheen Broth||BLU-10LET|
|4 ml Neutralizing Buffer||BLU-4NB|
|5 ml Neutralizing Buffer||BLU-5NB|
|10 ml Neutralizing Buffer||BLU-10NB|
|5 ml HiCap™ Neutralizing Broth||BLU-5HC|
|10 ml HiCap™ Neutralizing Broth||BLU-10HC|
|1 ml Letheen Broth, small swab||BLU-1LET-S|
|1 ml HiCap™ Broth, small swab||BLU-1HC-S|
|Dry, sterile swab||BLU-DRY|
PUR-Blue™ DUO Swab Sampler
PUR-Blue™ DUO Swab Sampler collects and enriches at point of collection. The swab comes pre-moistened with 1.5 ml of collection solution. The second side contains 9 ml of enrichment broth. After sample collection, the swab is immediately added to the broth to start sample enrichment.
|PUR-Blue DOU™ Swab, 100/case||Varenr.:|
|1.5 ml HiCap™ / 9 ml UVM||DUO-HC9UVM|
|1.5 ml HiCap™ / 9 ml Demi-Fraser Broth||DUO-HC9DFB|
|1.5 ml HiCap™ / 9 ml Buffered Peptone Water||DUO-HC9BPW|
|1.5 ml D/E / 9 ml UVM||DUO-DE9DFB|
|1.5 ml D/E / 9 ml Demi-Fraser Broth||DUO-DE9DFB|
|1.5 ml D/E / 9 ml Buffered Peptone Water||DUO-DE9BPW|
EZ Reach™ Sponge Sampler
The EZ Reach™ Sponge Sampler has a single flexible handle that detaches with an easy twist motion. Choose either a robust polyurethane or cellulose sponge and either a 24 oz or 18 oz sample bag.
|EZ Reach™ polyurethane, 100/case||Varenr.:|
|10 ml HiCap™ Neutralizing Broth||EZ-10HC-PUR|
|10 ml HiCap™ w/gloves||EZ-10HC-PUR-G|
|10 ml HiCap™ 18 oz bag w/gloves||EZ18FR-10HC-PUR|
|10 ml D/E Neutralizing Broth||EZ-10DE-PUR|
|10 ml Letheen Broth||EZ-10LET-PUR|
|10 ml Letheen Broth, 18 oz bag||EZ18FR-10LET-PUR|
|10 ml Neutralizing Buffer||EZ-10NB-PUR|
|10 ml Neutralizing Buffer w/gloves||EZ-10NB-PUR-G|
|10 ml Neutralizing Buffer 18 oz bag||EZ18FR-10NB-PUR|
|10 ml Neutralizing Buffer 18 oz w/gloves||EZ18FR-10NB-PUR-G|
|10 ml Buffered Peptone Water||EZ18FR-10BPW-PUR|
|Dry, sterole sponge||EZ-DRY-PUR|
|EZ Reach™ cellulose, 100/case||Varenr.:|
|10 ml Letheen Broth||EZ-10LET-CELL|
|10 ml Letheen Broth w/gloves||EZ-10LET-CELL-G|
|10 ml Neutralizing Buffer||EZ-10NB-CELL|
|10 ml Neutralizing Buffer w/gloves||EZ-10NB-CELL-G|
|10 ml Buffered Peptone Water||EZ-10BPW-CELL|
|25 ml Buffered Peptone Water||EZ-25BPW-CELL|
|Dry, sterile cellulose sponge||EZ-DRY-CELL|
|Adapters & Extension Poles||Varenr.:|
|EZ Reach™ Sponge Adapter for poles, each||EZ-ADAPT|
|PUR-Blue™ Swab Adapter for poles, each||BLU-ADAPT|
|4 ft Extension Pole, each||EXT POLE 4-FT|
|8 ft Extension Pole, each||EXT POLE 8-FT|
EZ Reach™ Split-Sampler
The EZ Reach™ Split-Sampler is our newest innovation, designed to deliver 2 samples form 1 collection site.
|EZ Reach™ Split-Sampler, 60/case||Varenr.:|
|20 ml HiCap™ Neutralizing Broth||EZ-SP-20HC-PUR|
|20 ml D/E Netralizing Broth||EZ-SP-20DE-PUR|
|20 ml Letheen Broth||EZ-SP-20LET-PUR|
|20 ml Neutralizing Broth||EZ-SP-20NB-PUR|
SampleRight™ Sponge Sampler
Our SampleRight™ Sponge Sampler is a traditional sponge sampling device for enviromental and carcass surfaces it includes a 1.5 x 3″ biocide-free polyurethane or cellulose sponge within a 18 oz or 24 oz sample bag. Each bag is printed with a white write-on area and is available with or without gloves attached.
|16 ml HiCap™ 18 oz bag, 100/case||EZ-SP-20HC-PUR|
|SampleRight™ cellulose, 100/case||Varenr.:|
|10 ml HiCap™ 18 oz bag||SR18-10HC|
|10 ml HiCap™ 18 oz bag w/gloves||SR18-10HC-G|
|10 ml Letheen Broth||SR-10LET|
|10 ml Letheen Broth w/gloves||SR-10LET-G|
|10 ml Letheen Broth, 18 oz bag w/gloves||SR18-10LET-G|
|10 ml Neutralizing Buffer||SR-10NB|
|10 ml Neutralizing Buffer w/gloves||SR-10NB-G|
|10 ml Neutralizing Buffer 18 oz bag w/gloves||SR18-10NB-G|
|10 ml Buffered Peptone Water w/gloves||SR-10BPW-G|
|10 ml Buffered Peptone Water 18 oz bag||SR18-10BPW|
|10 ml Buffered Peptone Water 18 oz w/gloves||SR18-10BPW-G|
|Dry cellulose sponge||SR-DRY|
|Dry cellulose sponge w/gloves||SR-DRY-G|
|Dry cellulose sponge, 18 oz bag||SR18-DRY|
|Dry cellulose sponge, 18 oz w/gloves||SR18-DRY-G|
Veriswab™ Swab Sampler
The Veriswab™ Sampler uses a Dacron tipped swab for reaching into small, hard to access areas.
|Veriswab™ Swab Sampler||Varenr.:|
|1 ml HiCap™ Neutralizing Broth||VS-1HC|
|10 ml Neutralizing Buffer||VS-10NB|
|4 ml Neutralizing Buffer||VS-4NB|
|10 ml Buffered Peptone Water||VS-10BPW|
|1 ml Letheen Broth||VS-1LET|
|4 ml Letheen Broth||VS-4LET|
|10 ml Letheen Broth||VS-10LET|
MWE Medical Wire
Wide range of applications throughout food and pharmaceutical manufacturing and processing
Food & Pharmaceutical Manufacturing
Sponge swabs are especially recommended for the sampling of larger areas for the detection of controlled pathogens such as Listeria, Salmonella and Coliforms. They are very useful for sampling irregular surfaces such as drains and machine casings, and also for larger areas such as conveyor belts and preparation benches. Polywipes™ with standard phosphate buffer has been widely used for many years, and is the most suitable formulation for most applications. There are some situations, whether specified by regulations, or the requirements of particular retailers, where alternative wetting solutions are required. To meet these requirements, Polywipes™ are now also available with Neutralising Rinse Solution (NRS™) , Buffered Peptone Water (BPW) or Letheen Broth. NRS™ is a peptone solution containing the neutralisers sodium thiosulphate, polysorbate 80, and lecithin, and is often specified for standard swab sampling. Buffered Peptone Water is recommended where Salmonella is a particular target on food contact surfaces. Letheen Broth is a growth medium which also contains the neutralisers lecithin and polysorbate 80, and is recommended by the FDA for environmental testing for the cosmetic industry. Peptone Saline (also called Maximum Recovery Diluent – MRD) is available as the wetting solution with Polywipes™ Carcass Swabs (see below).
European and international standards (including (EC) No. 2073/2005) require the regular sampling of the carcasses of cattle, sheep, pigs, goats and horses using sponge swabs. Polywipes™ Carcass Swabs are premoistened with Peptone Saline specially formulated (ISO 17604 & ISO 6887) to comply with these
regulations. They can be supplied complete with gloves, resealable bags, and preprinted label for recording required details such as Farm, Species, Length and Reference Number.
- l Range of premoistening buffers
- l Standard (Phosphate buffer)
- l NRS – neutralising rinse solution
- l Letheen Broth
- l BPW- Buffered Peptone Water
- l Peptone Saline (for carcass swabbing)
- l Sterile – guaranteed by irradiation
- l Triple packed for controlled handling
- l Bright blue sponge (highly visible – does not get lost)
- l Resistant to abrasion
- l Non-inhibitory – optimum recovery of target microorganisms
- l Premoistened with choice of buffers
- l Room temperature storage
- l Individually wrapped – tamper evident packaging
- l Variety of formats (gloves, bags, etc)
- l Scientifically attested
Premoistened sponge swabs for sampling large areas
Polywipes™ are sterile premoistened blue sponge swabs, ready to use and highly effective for the sampling and microbiological assessment of a wide variety of surfaces.
Polywipes™ are manufactured from a special sponge material containing no inhibitory substances, so that maximum recovery and detection of microorganisms is assured. The sponge format is convenient for sampling larger flat or irregular surfaces, such as conveyor belts and machine parts, as well as floors, walls, drains and ceilings.
Polywipes™ are premoistened with phosphate buffer, or any of a selection of other buffers, and individually sealed in a easy-to-open tamper evident peel pouch. The bright blue colour ensures they cannot be easily lost in critical areas, while the structure within the sponge prevents shedding of fragments due to abrasion.
Polywipes™ are also convenient to store at room temperature, and can be carried individually to the sites for testing. They are triple packed, and terminally sterilised by gamma irradiation.
Using sterile gloves, which can be supplied, the sponge is used to sample the surface being tested. Any traces of disinfectant are quickly neutralised, either by simple dilution, or by direct interaction with the neutralising components of the liquid. After use and transportation, they are readily processed in the laboratory by any of a number of standard techniques.
Polywipes™ have been the subject of many studies, including recovery performance with Salmonella, Listeria, MRSA,
Acinetobacter, and Clostridium difficile.
Neutralises trace disinfectant
NRS II Transwab® is MWE’s name for its range of swab based environmental sampling devices for the food, pharmaceutical, biotechnology and cosmetic industries. There can also be applications in healthcare including the monitoring of infection control measures.
All NRS II Transwab® devices feature MWE’s leak proof labelled self standing screw-cap tube made from shatterproof polypropylene, with a high visibility blue shaft swab attached to the cap. The swab features a rayon bud that can remain immersed in liquid, yet retains MWE’s high standard for absorption, survival and release of microorganisms. The tubes are prefilled with the specified volume of solution and there is a choice of NRS, MWE’s classic neutralising rinse solution, and a range of alternative buffers and media appropriate for particular applications.
NRS (Neutralising Rinse Solution)
NRS Medium contains lecithin, polysorbate 80, and sodium thiosulphate in a peptone phosphate buffer. The formulation conforms to ISO 18593 and will neutralise most disinfectants used in the food industry, including those based on chlorine, peroxygen compounds, amphoterics, biguanides, and glutaraldehyde. This is a universal neutralising solution suitable for testing most disinfected areas within the food, cosmetic and pharmaceutical industries. Precise fill volumes allow accurate quantitative assessment of contamination levels.
Buffered Peptone Water
Buffered Peptone Water contains peptone and sodium chloride, with a phosphate buffer, and is used for the recovery of Salmonella, especially in environments where they may have been sub-lethally injured.
Butterfield Buffer (also called Butterfield’s Solution or Butterfield’s phosphate buffered dilution water) contains potassium phosphate as specified by APHA and FDA, and is used in standard methods for the enumeration of bacteria and fungi in foods, water and pharmaceutical products. The standard formulation has been modified by the inclusion of a low leve one to reduce osmotic shock, and polysorbate 80 as a surfactant to assist the sampling process.
Letheen media are used to determine the bactericidal efficacy of quaternary ammonium based disinfectants. Letheen broth is a growth medium recommended by the FDA for use in the microbiological testing of cosmetics, and the formulation is as described in the FDA Bacteriological Analytical Manual. Polysorbate 80 and lecithin are included to partially neutralise the preservatives commonly found in cosmetics.
MRD (Maximum Recovery Diluent)
Also called Peptone Saline. Maximum Recovery Diluent is an isotonic and protective medium for maximum recovery of microorganisms from environmental and food sources. It is also the recommended diluent for the sampling of carcasses in the European Union.
Tryptic Soy Broth
Tryptic Soy Broth is a general purpose recovery medium with excellent growth properties for aerobes, anaerobes, and some fungi. It is widely used for sterility testing, particularly in pharmaceutical manufacturing.
NRS without Sodium Thiosulphate
Some testing methods (such as Petrifilm®) require medium without thiosulphate.
Choice of fill
NRS medium and variants are available in a choice of 3 fill sizes
- l 10ml and 5ml are used with standard and filtration methods.
- l 1ml can be used directly with Petrifilm®, and other direct pour plating techniques
For sterile manufacturing areas
SteriKit™ is a swab based system for the detection of microbial contamination in sterile production facilities.
Samples are collected using Steriswab™ premoistened swabs, and inserted into the SteriKit™ tubes containing an enriched semi-solid medium with colour change growth indicator. Upon incubation any bacteria will grow and cause the colour of the medium to change from purple to yellow within 48 hours.
The time taken will depend on the number of organisms, but in the event of any colour development, the swab and medium can be used for further investigation by conventional or molecular microbiological techniques.
- The product is triple wrapped to allow sterile handling in controlled areas, and sterilised by irradiation.
- Colour-change indication of microbial growth
- Enriched growth medium for enhanced detection for low numbers
- Premoistened swab for effective sampling
- Formulated and tested in accordance with UK, European & US Pharmacopoeias
- Ambient temperature storage
Longer flexible-shafted swab
Flexiswab™ is ideal for taps, pipes, vents, valves, vats, machinery, crevices, and other
- l Strong flexible longer 8 inch/ 205mm shaft for extra reach around bends without breaking.
- l High visibility blue shaft
- l Larger rayon bud for effective sampling with good absorption and release.
- l Tamper evident seal ensures sterility prior to sampling
- l Labelled tube for transport of sample, and recording site details
Sampling for critical surfaces
Steriswabs™ are designed for sampling of critical surfaces including nozzles and equipment in sterile production units
in hospitals and in the pharmaceutical industries. They are also particularly useful for surveillance of clean and food contact surfaces in the food manufacturing industry.
Steriswabs™ have a rayon bud on a blue plastic shaft, and are premoistened with a sterile phosphate buffer. The moistened bud improves the recovery of any microorganisms from the test surface, even if present in low numbers. The swabs have a 45mm breakp m to be readily transferred into other test systems.
Steriswabs™ are triple wrapped for sterile hand controlled premises, and are sterilised by irradiation.
PreMoist Swab Sampling Kits
PreMoist sampling swabs offer end-users a convenient and quick sampling method to conﬁrm the presence of bacteria with no additional preparation required. PreMoist Swabs can be used for environmental and hygiene monitoring, food security and cleaning validation due to the inclusion of neutralisers.
In many cases a surface will contain traces of disinfectants and/or sanitisers. Left alone and over time, these cleaning ﬂuid traces will not kill the microorganisms, but will just prevent any growth (bacteriostatic effect). TSC Neutralising Buffer will neutralise most common disinfectants including peroxides and derivatives, phenols, quaternary ammonium compounds (QAC’s), amphoterics, chlorines and associated halogens, providing end-users with total testing assurance.
Surface Sampling Swabs
- Easy direct sampling
- Choice of viscose and foam tips on white or blue polystyrene shafts
- Foam Tip Swab increases sample collection & release
- Deﬁne sampling areas by using a sterile sampling template [TS/15-T40]
- Labelled tubes for easier sample processing and identiﬁcation
- Tamper evident seals & shatterproof tubes offer total product conﬁdence
- Entire sample can be analysed
- Ideal for sampling a wide range of environments and critical surfaces
- Exposes potential microorganism contamination
- Useful in hygiene surveillance (HACCP) and environmental monitoring
- Food security / TACCP validation
- Protecting your brand and preventing costly recalls
Item 4513 – Cloth 32 x 40 cm, Tryptone salt at 10% neutralizing agent,
in blue sampling bag with pre-marked line at top for easy opening
Sterile device for surface sampling. For screening pathogens after disinfection in food processing industries, especially dairy.
- Blue non-woven cloth, honeycombed structure, size 32 x 40 cm
- Sampling bag l 19,5 x h 29,5 cm with pre-marked line at top for easy opening, one side blue for identification, one side clear
- Impregnation « TN »: 25 ml Tryptone salt at 10% neutralizing agent.
The neutralizing agent stops the inhibitory action of residual disinfectants and antiseptics (cf. Product data of Neutralizing agent). Calibrated dose
- Economical version: pack of 20 units in a zip bag, size 27 x 38 cm
- Box of 140 units (7 x 20 units)
- Dimensions of box approx. 40 x 30 x 30 cm
- Weight of box approx. 7 kg
Sterilization and traceability
- Sterilization by gamma rays dose 15 kiloGrays minimum
- Shelf life : 1 year from the date of sterilisation
- Batch number on each unit and on each box
- Sterilization certificate in each box