Lateral Flow Macadamia (Art. No. BLH705-15) with included hook line from Bioavid is an immunochromatographic test for sensitive and qualitative detection of Macadamia residues on surfaces (e.g. swab test for hygiene control in food production lines), in cleaning/process water (CIP water) and food samples. The integrated hook line prevents false negative interpretations of highly positive samples.
Available on backorder
To avoid allergic reactions, it is ensured that foods are labeled with the content of allergenic ingredients. Nevertheless, there is still a risk of unintentional presence of allergens due to cross-contamination during processing. It is therefore important to avoid and detect cross-contamination (e.g. due to residues on production lines). Bioavid Lateral Flow Macadamia rapid tests can be used for various purposes to detect contamination. For example, incoming raw materials or the effectiveness of the cleaning of production facilities can be checked.
According to Regulation (EU) No 1169/2011, Macadamia and its products must be declared on foods.
A key challenge in safe and fast lateral flow analysis is false negative results due to very high allergen concentrations in the sample. The so-called “high dose hook effect” (or “overload effect”; Standard EN 15633-1. 2019) occurs when a very high amount of an analyte is present in the sample. In this case, the development of the test line can be inhibited and there is a risk that the test result is wrongly assessed as negative. The hook line on the test strip makes this effect visible. A missing hook line indicates a high allergen content in the sample and can no longer be assessed as negative. The evaluation chart for Bioavid test strips with hook line can be downloaded here.
Bioavid lateral flow sticks are an easy method that enables fast and reliable analysis. The application is very simple and can also be performed by personnel without laboratory training. The processing of all Bioavid parameters is identical and can be downloaded here as a flyer. All test kits can be stored at room temperature and are therefore an ideal tool for direct testing in production facilities or for rapid food testing.
Strongly colored (e.g. coffee, tea, spices, wine), polyphenol/tannin containing, oily or acidic/alkaline samples (e.g. juices) should be extracted with Absorbent Buffer (Art. No. BS810-25). The buffer decolorizes or neutralizes solutions to avoid false positive results and minimize matrix effects.
Specifications: Lateral Flow Macadamia incl. Hook line
| Product number | BLH705-15 |
| Test format | 15 test strips (15 provisions) |
| Test preparation | – Surfaces: wet the swab in the tube with PBS. Swab the surface and clean the swab in the same tube. – Product samples: homogenize the sample. Dissolve in distilled water, centrifuge or filter – Cleaning water (e.g. CIP water: no sample preparation |
| Incubation period | 5 min. at room temperature 2 – 25 °C (35 – 77 °F) |
| LOD (Limit of Detection) | (CIP) water: 0.5 µg/mL (ppm) Surfaces (swabs): 0.1 µg/cm2 Product samples: down to 5 mg/kg (ppm)* *dependent on matrix |
| Cross reactions | Hazelnut 0.5% |
| Allergen detected | Macadamia nut |
| Reading | Visual |
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